Journal: Scientific Reports

Article Title: Developmental timing of auditory deprivation influences spatial memory and hippocampal plasticity in rats

doi: 10.1038/s41598-025-26704-8

Figure Lengend Snippet: ( A ) Western blot analysis representative image bands of BDNF, synaptophysin, and PSD-95 in the hippocampus ( B ) Quantitative representation of the protein expression levels in the hippocampus, normalized to β-actin. Data are represented as mean ± SD. * P < 0.05, ** P < 0.01, *** P < 0.001 show the comparison between control and CA groups and # P < 0.05, ## P < 0.01, and ### P < 0.001 show the comparisons between the groups. No splicing or image manipulation was performed. The PVDF membranes were not cut before antibody incubation. Only grayscale conversion and uniform brightness and contrast adjustments were applied to improve clarity. Full-length blots are available in Fig. S2.

Article Snippet: The membranes were blocked using bovine serum albumin (BSA) in 0.1% Tween 20 for 1 h. Then, the membranes were incubated overnight at 4oC with specific primary antibodies: rabbit anti- BDNF antibody (orb10181, Biorbyt, Wuhan, China, 1:300), rabbit Anti-PSD95 antibody (orb500730, Biorbyt, Wuhan, China, 1:1000), rabbit anti-Synaptophysin antibody (orb704356, Biorbyt, Wuhan, China, 1:600), and rabbit anti-beta Actin antibody (GTX109639, Genetex, USA, 1:1000).

Techniques: Western Blot, Expressing, Comparison, Control, Incubation

Journal: Pharmaceutics

Article Title: Anti-Neuroinflammation Effect of Standardized Ethanol Extract of Leaves of Perilla frutescens var. acuta on Aβ-Induced Alzheimer’s Disease-like Mouse Model

doi: 10.3390/pharmaceutics17081045

Figure Lengend Snippet: The effect of PE on LPS-induced changes in CREB phosphorylation and BDNF expression in BV2 microglial cells. BV2 microglial cells were pretreated with PE for 2 h, followed by stimulation with LPS (50 ng/mL). Protein expression was analyzed by Western blot. ( A ) pCREB expression normalized to total CREB. ( B ) mature BDNF expression normalized to β-actin. Data are presented as means ± SDs. Statistical analysis was performed using one-way ANOVA followed by the Newman–Keuls post hoc test. # p < 0.05, ### p < 0.001 vs. normal (NOR) group; * p < 0.05, ** p < 0.01, and *** p < 0.001 vs. LPS-treated control (CON) group.

Article Snippet: Rabbit monoclonal BDNF antibody was obtained from Cusabio (Houston, TX, USA), and rabbit polyclonal antibodies against histone H3 and PARP1 were purchased from Epitomics (Abcam, Waltham, MA, USA).

Techniques: Phospho-proteomics, Expressing, Western Blot, Control

Journal: Pharmaceutics

Article Title: Anti-Neuroinflammation Effect of Standardized Ethanol Extract of Leaves of Perilla frutescens var. acuta on Aβ-Induced Alzheimer’s Disease-like Mouse Model

doi: 10.3390/pharmaceutics17081045

Figure Lengend Snippet: Effect of PE on Aβ-induced changes in pCREB/BDNF signaling. Western blot analysis was performed on hippocampal tissue from Aβ-injected mice to assess the effect of PE on CREB/BDNF signaling. Mice were administered PE (500 mg/kg, p.o.) once daily for 7 d after Aβ injection. ( A ) pCREB normalized to total CREB. ( B ) BDNF expression normalized to GAPDH. Data are presented as means ± SDs. Statistical analysis was performed using one-way ANOVA followed by the Newman–Keuls post hoc test. # p < 0.05 vs. normal (NOR) group; * p < 0.05 vs. Aβ-injected control (CON) group.

Article Snippet: Rabbit monoclonal BDNF antibody was obtained from Cusabio (Houston, TX, USA), and rabbit polyclonal antibodies against histone H3 and PARP1 were purchased from Epitomics (Abcam, Waltham, MA, USA).

Techniques: Western Blot, Injection, Expressing, Control